Gallus gallus Ovarian Raw sequence reads

In order to screen differential expressed genes between transcriptome of high and low egg production chicken ovaries, RNA sequencing was carried out. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups of proteins (COG) were performed on new genes and DEGs. 13 DEGs were randomly selected to conduct RT-PCR to verify the RNA-seq results. Our study can not only provided a theoretical basis for regulatory mechanism research of egg production, but also provided candidate genes for genetic improvement of egg production.为筛选高产蛋鸡与低产蛋鸡卵巢转录组中的差异表达基因，本研究开展了RNA测序实验。针对新基因与差异表达基因（differentially expressed genes，DEGs），本研究进行了基因本体论（Gene Ontology, GO）、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）以及蛋白质直系同源簇（Clusters of Orthologous Groups of proteins, COG）富集分析。随机选取13个DEGs开展逆转录聚合酶链式反应（Reverse Transcription-Polymerase Chain Reaction, RT-PCR）实验，以验证RNA测序结果的准确性。本研究不仅为产蛋性状调控机制的研究提供了理论基础，同时也为鸡产蛋性状的遗传改良提供了候选基因。