Metabolic Reprogramming in hnox Paracoccus denitrificans Revealed by Time-Resolved Metabolomics and Artificial Intelligence-Driven Proteome Modeling

Heme-nitric oxide/oxygen binding (H-NOX) proteins function as critical sensors for nitric oxide in many bacterial species. However, their physiological functions are surprisingly diverse, and most have yet to be thoroughly investigated. Here, we investigate the impact of hnox deletion in Paracoccus denitrificans, a species known for its metabolic versatility and the formation of unusually thin biofilm structures.