Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12158, Gallus gallus gallus, memory B cell. CIL. Dataset

Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is a central z-slice. Localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A(green) in INCENP-OFF cells expressing TrAP-INCENP-W766G. Chromosomes stained with DAPI (blue). See Fig 4F panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系细胞在有丝分裂期的细胞。利用Deltavision系统，通过100x/1.4NA计划阿波 objective 镜头采集的宽场免疫荧光图像的Z系列，采用约束迭代算法（Applied Precision）进行去卷积处理。展示的是中央的Z切片。在表达TrAP-INCENP-W766G的INCENP-OFF细胞中，外源性的TrAP-INCENP（红色）与内源性的CENP-A（绿色）定位。染色体用DAPI（蓝色）染色。参见Xu等人在2009年《细胞生物学杂志》第187卷第637-653页的图4F面板1。