Effects of endurance flight on mitochondrial physiology, lean mass dynamics and flight muscle morphology in blackpoll warblers

Migratory birds are physiologically challenged by intense exercise while fasting during flights that may last hours to days. Exercise-induced oxidative stress could compromise flight performance by inducing mitochondrial dysfunction in the flight muscle. Endurance flight is partially fuelled by the catabolism of lean tissues, but how this catabolism is partitioned between different organs and muscles has not been previously studied under controlled conditions. We hypothesized that simulated migratory flight would result in dysfunction of flight muscle mitochondria, and selective catabolism of lean tissues. We predicted that simulated migratory flight would cause reduced mitochondrial oxidative phosphorylation capacity while increasing emission of reactive oxygen species (ROS) and that lean tissue mass catabolism would preferentially occur in digestive organs not needed in flight. We measured mitochondrial function, muscle morphology and the wet masses of organs and muscles following 8-h..., , , # Effects of endurance flight on mitochondrial physiology, lean mass dynamics and flight muscle morphology in blackpoll warblers [https://doi.org/10.5061/dryad.s4mw6m9hc](https://doi.org/10.5061/dryad.s4mw6m9hc) ## Description of the data and file structure Blackpoll warblers (*Setophaga striata*) were split into three experimental groups and each was fasted for one hour prior to each group's intervention: 1. Flown: birds were sampled after being flown 7.5-8 hours in a wind tunnel 2. Fasted: birds were sampled after being fasted from food and water for 7.5-8 hours in the wind tunnel antechamber 3. Pre-flight: birds were sampled following the 1 hour fast Body composition for flown and fasted birds was measured before and after flight/fasting using quantitive magnetic resonance imaging. After euthanasia, multiple tissues were harvested and weighed. Some flight muscle was embedded for histological analyses, while remaining muscle was used to isolate mitochondria. High-resolution respi...# 耐力飞行对黑顶林莺（Blackpoll warbler，学名*Setophaga striata*）线粒体生理、瘦体重动态及飞翔肌形态的影响 候鸟在持续数小时至数天的飞行过程中，需同时承受高强度运动负荷与禁食状态带来的双重生理挑战。运动诱导的氧化应激可通过引发飞翔肌线粒体功能障碍，损害飞行表现。耐力飞行的部分能量来源于瘦组织的分解代谢，但此前尚未有受控实验条件下的研究阐明该分解代谢在不同器官与肌肉间的分配模式。本研究提出假设：模拟迁徙飞行会导致飞翔肌线粒体功能障碍，并引发瘦组织的选择性分解代谢。我们预测，模拟迁徙飞行会降低线粒体氧化磷酸化能力，同时提升活性氧（reactive oxygen species, ROS）的释放量，且瘦组织质量的分解代谢会优先发生于飞行过程中非必需的消化器官。我们对8小时干预后的线粒体功能、肌肉形态以及器官与肌肉的湿重进行了检测…… [https://doi.org/10.5061/dryad.s4mw6m9hc](https://doi.org/10.5061/dryad.s4mw6m9hc) ## 数据与文件结构说明 黑顶林莺（*Setophaga striata*，Blackpoll warbler）被分为3个实验组，每组均在干预前禁食1小时： 1. 飞行组：在风洞内完成7.5~8小时飞行后对受试鸟类进行采样 2. 禁食组：在风洞前室内禁食食物与水源7.5~8小时后对受试鸟类进行采样 3. 飞行前组：完成1小时禁食后即刻对受试鸟类进行采样 研究人员采用定量磁共振成像分别检测飞行组与禁食组鸟类在飞行/禁食前后的身体成分。受试动物安乐处死后，采集多种组织并称重。部分飞翔肌样本被包埋以用于组织学分析，剩余肌肉样本则用于线粒体分离。高分辨率呼吸……