m6A-seq for testis in Xia-Nan cattle

Xia-Nan (XN) cattle, the first specialized beef breed crossbreeding French Charolais (male) and Nanyang cattle (female) in China, has important features such as a fast growth rate and high meat production performance. To identify the functional m6A and explore the spermatogenesis mechanism of the testes caused by the m6A RNA modification, we detected the m6A methylomes of bovine testes from birth to adulthood. This enabled us to obtain the transcriptome-wide m6A profiles in bovine testes at different stages and to provide reasonable insights into the roles of m6A modification in the mechanisms underlying spermatogenesis during post-natal testicular development.