RNA-Seq of Notch-stimulated overgrowing Drosophila melanogaster wing imaginal discs

Aim: mRNA profile of larval wing imaginal discs of Drosophila melanogaster to study the cooperation between Notch activation and loss of epithelial polarity (scrib mutation) during neoplastic growth. Results: The combination of Notch activation and scribble mutation (NS) results in mRNA expression changes that, while partly overlapping with Notch only (N), and with scrib mutation only (S), are unique to the combination Overall design: Triplicates of pooled dissected 3rd instar larva wing imaginal discs (60 discs in each pool). All dissections at day 6 after egg laying for the following conditions: wild type (WT), activated Notch (N), polarity deficient (scrib1 mutant, S), activated Notch and polarity deficient (NS)