m6A epitranscriptomic changes in response to hypoxic condition or ALKBH5 knock-down in U87

Compared with the normoxia control, 174 m6A peaks from 58 m6A-modified transcripts are significantly increased while 2032 m6A peaks from 556 m6A-modified transcripts are significantly decreased in hypoxia. Among the downregulated m6A peaks in hypoxia compared with normoxia condition, ALKBH5 depletion leads to 847 increased m6A peaks in 276 transcripts (fold change≥1.5). The m6A profiles of U87 WT-Normoxia, U87 WT-Hypoxia, U87 shScr-Hypoxia and U87 shALKBH5-Hypoxia