Single-cell RNA-seq of astrocytes and neurons from primary culture of the mouse brain and of acute slice of interneurons from Patch-clamp pipette [RNA-seq mouse brain]

To investigate the relationship between single-cell transcriptional activity and single-strand open-chromatin landscape in mouse brain, we performed single-cell RNA-seq. Overall design: We did single-cell RNA-seq for 8 single astrocytes and 12 single neurons from mouse brain primary cultures. We also did single-cell RNA-seq for 8 interneurons collected from acute slice of mouse brain using Patch-clamp pipette.