RIP-seq using ILF3 antibodies to reveal RNAs that binds to ILF3 protein
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https://www.ncbi.nlm.nih.gov/sra/SRP299258
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资源简介：
RNA pulldown assay showed that lncRNA UBE2CP3 could bind to ILF3 protein. In order to further verify the interaction between ILF3 and UBE2CP3, RNA Immunoprecipitation (RIP) assay was performed to identify the RNAs that binds to ILF3 protein. RIP-seq data verifies the interaction between ILF3 and UBE2CP3. Interestingly, UBE2CP3 could also binds to 3'UTR of IGFBP7 mRNA. Mechanismly, lncRNA UBE2CP3 and 3' UTR of IGFBP7 could forms an double-stranded RNA. Then, the RNA duplex could interact with the Double-Stranded RNA-Binding Protein ILF3. Overall design: The potent RNA was immunoprecipitated by ILF3 antibodies using RIP assay. Then, all the potent ILF3-binding target RNAs were identified by deep sequencing, using Illumina HiSeq 4000.
创建时间：
2021-07-21
