FaNUDT19 contributes to 5′ cap quality control by modulating NAD+ capped RNA dynamics during strawberry fruit ripening

NAD+ capped RNAs (NAD-RNAs) are widely present in prokaryote and eukaryote transcriptomes. However, their regulation during developmental processes are largely unknown. In this study, we investigated the regulation of NAD-RNAs during receptacle ripening in the strawberry (Fragaria × ananassa, Fa), a model for non-climacteric ripening. To do this, we performed “click reaction”-based method to sequence NAD-RNAs (SPAAC-NAD-seq) to globally profile NAD-RNAs in the context of strawberry ripening. From this analysis, we identified 9,109 NAD-RNAs produced from protein-coding genes (NAD-mRNAs), 6,479 from transposable elements (NAD-TE-RNAs), 4 from the mitochondrial genome, and 11 from the chloroplast genome. We also found that the number of NAD-RNAs displayed an overall decreasing trend that corresponded with lower abundance of NAD+ cap throughout the course of ripening. Concurrently, we characterized the function of FaNUDT19, a potent NAD-RNA scavenger, in strawberry fruit ripening. Correspon..., , # FaNUDT19 contributes to 5′ cap quality control by modulating NAD+ capped RNA dynamics during strawberry fruit ripening Dataset DOI: [10.5061/dryad.rbnzs7ht0](https://doi.org/10.5061/dryad.rbnzs7ht0) ## Description of the data and file structure This is the original phylogenetic analysis data in Newick form of NudC homologs. ### Files and variables #### File: NudC_homolog_align.nwk **Description:** This is the original phylogenetic analysis data in Newick form of NudC homologs. This file can be read by MEGA software. #### File: NudC_homolog-align.txt **Description:** This is the original alignment in .txt form of NudC homologs. #### File: Aligned.praln **Description:** This is the original alignment in .praln form of NudC homologs. This file can be read by Snapgene software. #### File: NudC_seq_for_align.txt **Description:** This is the sequences of NudC homologs for alignment. #### File: NudC_seq_for_tree.txt **Description:** This is the sequences of NudC homologs for ph..., ,NAD+加帽RNA（NAD+ capped RNAs，简称NAD-RNAs）广泛存在于原核生物和真核生物的转录组中。然而，其在发育过程中的调控机制仍未被充分阐明。本研究以非呼吸跃变型果实成熟模式植物栽培草莓（Fragaria × ananassa，简称Fa）的花托成熟过程为研究对象，探究了NAD-RNAs的调控机制。为此，我们采用基于"点击化学反应"的NAD-RNA测序方法（SPAAC-NAD-seq），在草莓成熟的整体背景下对NAD-RNAs进行全局图谱分析。通过该分析，我们共鉴定到9109个来自蛋白编码基因的NAD-RNA（简称NAD-mRNAs）、6479个来自转座因子的NAD-RNA（简称NAD-TE-RNAs）、4个来自线粒体基因组的NAD-RNA以及11个来自叶绿体基因组的NAD-RNA。我们还发现，在草莓成熟的整个过程中，NAD-RNA的数量整体呈下降趋势，这与NAD+加帽的丰度降低相吻合。与此同时，我们对草莓果实成熟过程中高效NAD-RNA清除剂FaNUDT19的功能进行了表征。相关研究表明……# FaNUDT19通过调控草莓果实成熟过程中NAD+加帽RNA的动态变化，参与5'端加帽质量控制 数据集DOI：[10.5061/dryad.rbnzs7ht0](https://doi.org/10.5061/dryad.rbnzs7ht0) ## 数据与文件结构说明 本数据集包含NudC同源蛋白的Newick格式原始系统发育分析数据。 ### 文件与变量 #### 文件：NudC_homolog_align.nwk **文件说明**：该文件为NudC同源蛋白的Newick格式原始系统发育分析数据，可通过MEGA软件读取。 #### 文件：NudC_homolog-align.txt **文件说明**：该文件为NudC同源蛋白的.txt格式原始多序列联配数据。 #### 文件：Aligned.praln **文件说明**：该文件为NudC同源蛋白的.praln格式原始多序列联配数据，可通过Snapgene软件读取。 #### 文件：NudC_seq_for_align.txt **文件说明**：该文件包含用于多序列联配分析的NudC同源蛋白序列。 #### 文件：NudC_seq_for_tree.txt **文件说明**：该文件包含用于系统发育分析的NudC同源蛋白序列……