Epigenetic Mechanisms Regulating The Transcription of BBB Genes: Role of Wnt/ß-catenin signaling

To identify CNS endothelial cell (EC) the gene cohorts that participate in blood brain barrier (BBB) formation versus maintenance. We investigated mechanism behind the reduced Wnt/ß-catenin signaling observed in adult CNS ECs We also investigated whether the adult endothelial cells can re express the EC gene cohorts required for the BBB formation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for E-13.5 cortical ECs vs Adult Cortical ECs, vs Wnt3a (200nM) treated Adult cortical Ecs, Adult cortical ECs versus MS-275 (10uM) treated cortical Ecs.