Gene expression in Human Bronchial Epithelial Cells under Differing Oxygen Regimes

Bronchial epithelial cells line the small airways bronchii and small airways of the lungs, acting to trap and remove contaminants deposited in the lung through the process of inhalation. These cells are sensitive decreased oxygen concentrations, and are invoved in local signaling in hypoxic conditions, such as when airways are occluded. We grew cultured human bronchial epithelial cells for three passes, and treated the fourth pass under three different oxygen concentrations (21%, 15%, 2%). RNA from each of three replicates of each treatment was extracted, processed, and hybridized onto Affymetrix Human Transcriptome Array 2.0 microarrays to examine alterations in gene expression under these oxygen regimes. This data was collected to complement ATAC-seq data from cells aliquotted from each replicate. Primary Human Bronchial epithelial cells from the same individual were grown under standard conditions (5% CO2, 37C, 100% humidity) for three passes, and trasnferred during the fourth pass to 6-well plates for treatment. Treatment proceeded in a controlled-oxygen environmental chamber at 2%, 15%, or 21% oxygen. Three biological replicates were obtained per treatment, each of which were prepared and analyzed separately.