Microbial Bacterial Production in Lakes at North Temperate Lakes LTER 2000 - 2002
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Net production of bacteria passing a 70 micron mesh, or bacteria passing a 1 micron
mesh, calculated incorporation of 3H labeled leucine into cell proteins. Method based on
microcentrifuge method of Smith, David C., and Farooq Azam. 1992. A simple, economical
method for measuring bacterial protein synthesis rates in seawater using 3H-leucine, Marine
Microbial Food Webs 6(2):107-114. Nanomolar treatments refer to leucine concentration for
calculation of isotopic dilution and necessary leucine concentration to saturate uptake into
bacterial cells. For a good example on how to calculate isotopic dilution see Pace. Michael
L., and Jonathan J. Cole. Primary and bacterial production in lakes: are they coupled over
depth? Plankton Research 16(6):661-672. Sampling Frequency: fortnightly during ice-free
season - every 6 weeks during ice-covered season Number of sites: 4
创建时间:
2013-06-14



