Energy allocation explains how protozoan phenotypic traits change in response to temperature and resource supply

To survive and reproduce, living organisms need to maintain an efficient balance between energy intake and energy expenditure. Changes in environmental conditions can disrupt previously efficient energy allocation strategies, and organisms are required to change their behaviour, physiology, or morphology to cope with the new environment. However, how multiple phenotypic traits interact with one another and with environmental conditions to shape energy allocation remains poorly understood. To better understand this type of phenotype-environment interactions, we develop a predictive framework, grounded in energetic and biophysical principles that allows us to make predictions on how metabolic rate and movement speed should change in response to environmental temperature and resource supply, differentiating between short-term, acute exposure to novel conditions and longer-term exposure that allows acclimation or adaptation. We tested these predictions by exposing axenic populations of the ..., Cell cultures
Axenic cultures of Tetrahymena pyriformis strain 1630/1W were obtained from CCAP (www.ccap.ac.uk) and cultured at 20 degrees Celsius in a Proteose peptone - yeast extract medium 20 g Proteose peptone + 2.5 g yeast extract in 1L deionized water. Cells were maintained in the exponential growth phase at 20°C and 100% medium concentration for about 50 days (constant temperature, no light) in two replicates. Subsequently, cultures were split across 9 different treatments (3 temperature conditions x 3 medium concentrations) and adapted to the new conditions for at least three weeks (corresponding to a minimum of 20 generations), in four replicates for each condition.
Throughout the entire duration of the adaptation period, cultures were kept in relatively constant growing conditions by adopting a serial transfer regime, subculturing repeatedly into fresh growth medium. At the end of the adaptation period, cultures were further split across a wide range of temperature conditions ..., , # Energy allocation explains how protozoan phenotypic traits change in response to temperature and resource supply

[https://doi.org/10.5061/dryad.2v6wwpzvv](https://doi.org/10.5061/dryad.2v6wwpzvv)

Please refer to the original manuscript for details of methods. The code provided is meant to directly read the CSV data files with column names and other information as provided. 

## Description of the data and file structure

File: Tetrahymena-revised.zip

Video tracking and analysis of the movement of *Tetrahymena pyriformis*

This repository contains the code (mostly R) associated with the manuscript

[https://doi.org/10.1101/2023.07.25.550219](https://doi.org/10.1101/2023.07.25.550219)

The data are also in the repository.

The **video_tracking folder** contains the Python scripts used for tracking *Tetrahymena* cells from videos recorded under the microscope. If the scripts are used on different videos, it might be necessary to change file paths and also some parameters, such as the ...,