Divergent principles specifying inflammatory versus antiviral transcriptional programs in dendritic cells [ATAC-seq]

Stimulus-specific gene expression programs are enabled by enhancers, on which stimulus-regulated transcription factors (SRTFs) can land in a cell type- and stimulus-dependent manner. In this study, we identified the key features of enhancers that mediate differential responses to Toll-like receptor (TLR)-stimulation. We characterized the TLR3- and TLR9-induced programs and enhancers in CD8+ dendritic cells.The relevance of these features has been confirmed via machine learning application and by mapping SRTF-binding. Overall design: CD8-positive dendritic cells were left unstimulated or were treated for the indicated periods of time (6h or 90 mins) with 5 mg/ml of high molecular weight Polyinosinic-polycytidylic acid (pIC) or 1 mM class B CpG oligonucleotide 1826 (CpG).