Comparison among samples subjected to a novel in vitro tissue culture system, VISUAL-CC. Comparison among samples subjected to a novel in vitro tissue culture system, VISUAL-CC

We established a novel in vitro tissue culture system (named VISUAL-CC), in which phloem companion cell (CC) differentiation can be induced with Arabidopsis thaliana cotyledons. To compare gene expression profiles between VISUAL and VISUAL-CC, we conducted GeneChip analysis using two different in vitro cultures. CC-S means a sample that strongly induces CC differentiation. CC-M means a sample that moderately induces CC differentiation. V means a VISUAL sample, that does not induce CC differentiation at all. Overall design: Arabidopsis cotyledons cultured with the CC-induction medium were sampled at 96 hours after the induction.