Effect of L-glutamine on the metabolism of deadherent cancer cells.

MCF-7 cells transfected with osteopontin-c (C) were plated in poly-HEMA coated wells in complete medium (2 mM L-glutamine) or low glutamine medium (0.5 mM). MCF-7 vector cells (V) were plated in poly-HEMA coated wells in complete medium (2 mM L-glutamine) or high glutamine medium (4 mM). After 48 hours, the cells were harvested and lysed for analysis of the metabolite levels by NMR. Shown are comparison of MCF-7 OPNc to MCF-7 vector, both at normal levels of L-glutamine (2 mM), comparison of MCF-7 OPNc normal to low L-glutamine, comparison of MCF-7 vector normal to high L-glutamine.Effect of L-glutamine on the metabolism of deadherent cancer cells.