Cross-species animal-level pseudobulk analysis of mitochondrial transcriptional responses associated with prolonged sevoflurane exposure in the aged hippocampus: analysis code and derived results

This record contains the analysis code, frozen gene-set definitions, accession-to-sample mappings, input hashes, one-to-one mouse-marmoset ortholog mappings, validation gates, software environment information, derived result tables, and output manifests supporting the study “Cross-species animal-level pseudobulk analysis of mitochondrial transcriptional responses associated with prolonged sevoflurane exposure in the aged hippocampus.” The primary analysis treats animals rather than cells as biological replicates and evaluates 13 predefined gene sets in five shared cell types across GSE222430 and GSE234493. GSE303920 is included only as a same-animal alternative-processing sensitivity analysis, and GSE155770 is included only as a high-risk bulk boundary analysis. The release includes the complete 65-hypothesis primary family, matched-random-set evaluations, top-five gene deletion analyses, leave-one-animal-out analyses, and sensitivity tables including negative and non-testable results. Raw GEO expression matrices are not redistributed and remain available from their original accession records. This deposit supports reproducibility of the computational analyses. Its RNA-level results do not establish mitochondrial functional change, ferroptosis, neurotoxicity, or causality. Licensing is component-specific rather than an umbrella license for the archive: analysis scripts are under the MIT License; original documentation, figures, and derived statistical result tables are under CC BY 4.0; FerrDb-derived frozen content remains under CC BY-NC 4.0; and all other third-party-derived materials retain their source terms. See `LICENSE-CODE.txt` and `LICENSE-DATA.txt` for the exact scopes.