RNA-seq analysis of substates of human pluripotent stem cells with mesoderm bias and early differentiating cells towards the mesoderm lineage

The differentiation of human pluripotent stem cells (hPSC) towards clinically relevant cells for regenerative medicine has been hampered by poor differentiation efficiencies. This may be a result, in part, of the heterogeneity that exists within stem cell cultures. We have generated a new stem cell medium PRIMO, which biases cells towards mesodermal differentiation without losing pluripotency. This experiment characterizes the gene expression of cells grown in PRIMO and compares this to cells grown in standard culture conditions and a 72 hour time course of mesodermal differentiation