Dataset in support of the thesis 'An ultra-high-speed imaging study of ultrasound-mediated mechanotransdcution'

Dataset in support of the thesis "An ultra-high-speed imaging study of ultrasound-mediated mechanotransduction"

The data includes the following data for the three technical chapters:

Chapter 3 - IBUCS test:

cross scratch images:
- data from 3 cross scratch samples images with and without phase contrast at 500kfps

device viability data:
- Three time point images of cells stained with Calcein
- One data sheet with raw values from the PrestoBlue cell viability test

PMMA calibration:
- Raw data from PMMA calibration - 3 samples without PDMS well and 3 samples with PDMS well
- Includes image data for strain analysis and IR data
- Samples with PDMS well were imaged on well side and non-well side.
- Each dataset has images from different sonotrode powers - 10%, 20%, 30%, 40% and 50%

Chapter 4 - Quantifying mechanical responses:

cell and scratch images:
- high-speed images of cell deformation for three cell/scratch datasets, all imaged at 500kfps

fluorescent imaging:
- high-speed images of cell deformation of four sets of cells stained with fluorescent markers, all imaged at 500kfps

inertial environments:
- high-speed images of monolayer deformation (monolayers A-I), all imaged at 500kfps

single cell vs monolayer:
- high-speed images of monolayer 1-4 deformation and single cell 1-4 deformation, all imaged at 500kfps

Chapter 5 - Mechanotransduction and ultrasound:

alizarin red:
- destain absorbance readings for alizarin red experiments.

pcr:
- raw data for samples for each set of genes tested at 10 day timepoint.

viability tests:
- absorbance readings from viability tests at 20% and 40% excitation for range of time points.
- associated thermocouple raw data from stated viability tests.

This work is funded by the Engineering and Physical Sciences Research Council and part of the Ultrasurge Project under Grant EP/R045291/1.