Neuroprotective effect of miR-204 OE in mouse models of Inherited Retinal Disease (IRD)

Transcriptome profile of retinas from wild-type (c57BL/6) and RHO-P347S mice following AAV-mediated subretinal delivery of pre-miR-204 to study the molecular changes underlying the neuroprotective effect of miR-204 OE in mouse models of Inherited Retinal Disease (IRD) To determine the molecular changes associated with AAV-mediated delivery of miR-204 in the subretinal space of wild-type and IRD mouse models, we performed RNASeq analysis of retinas from c57BL/6 and RHO-P347S mice injected with the AAV2/8.CMV.miR204 and AAV2/8.CMV.miR204MUT vector in a paired manner. Overall design: Total RNA was extracted at postnatal day (P) 12 from retinas injected at P4 with the AAV2/8.CMV.miR204 vector; RNA extracted from retinas of controlateral eyes (injected with the control). Designation of contralateral eyes i.e. OS=Left eye, OD=Right eye of the same animal AAV2/8.CMV.miR204MUT vector) was used as control.