Analysis of the nitrate-responsive transcriptome during root nodule symbiosis in wild type, the Ljnin mutants, the nrsym1 mutants, the nrsym2 mutants, and the nrsym1 nrsym2 double mutants of Lotus japonicus

To gain insights into the roles of NRSYM1 and NRSYM2 in the nitrate-responsive gene expression in Lotus japonicus, we conducted RNA-seq of the roots of wild-type (MG-20), the nrsym1-1 mutants, the nrsym2 mutants, and the nrsym1-1 nrsym2 double mutants grown with or without nitrate during root nodule symbiosis. We performed RNA-seq analysis of the roots of wild-type and the Ljnin-9 mutants grown with or without rhizobia to identify LjNIN-dependent rhizobia-inducible genes.