Drosophila melanogaster wing images from genetic manipulations of planar cell polarity and tissue growth genes (UAS-genex.RNAi and NP6333-GAL4)

These are images of <i>Drosophila melanogaster </i>wings from RNAi knockdowns (using VDRC RNAi lines) crossed to the NP6333-GAL4 line (also known as the <i>Pen</i>-GAL4 line). These crosses (and images) were done just to test the lines. The UAS-<i>narrow</i>.RNAi did not seem to work, but all the other ones did.<br>Crosses were performed by Runyang Yin in the Dworkin lab at McMaster University.<br>All crosses were done at 24°C in a Percival Growth Chamber (12:12 light cycle). Adult flies were stored in 70% ethanol prior to dissection in 70% glycerol/PBS (with a drop of phenol as a bacterio-static agent).<br><br>All images were taken on an Olympus BX-43 microscope with a 4X objective (40X total magnification). The objective is UPlanSApo (NA 0.16. FN26.5). Images were captured on an Olympus DP80 digital camera using Olympus cellSenStandard software (v1.14). <br><br>Image resolution pixels: 4080 x 3072Image size actual: 2.2 mm x 1.7 mm537.5 nm/pixel<br>24bit RGB (8 bit/channel)<br>genes manipulated<i>fat</i><i><br></i><i>narrow</i> (not really PCP or growth, but has an effect on morphogenesis). Note this construct did not seem to do much, so these pictures may not really be a manipulation of <i>narrow</i>. We re-ordered it and the new strain had much clearer effects.<br><i>starry night</i> (<i>stan</i>)<i>Van Gogh</i> (<i>vang</i>)<br><br>