Data from: First molecular characterization of Echinococcus granulosus (sensu stricto) genotype 1 among cattle in Sudan

Background: Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis (CE), which is a cosmopolitan zoonotic parasitic disease infecting humans and a wide range of mammalian species including cattle. Currently, little information is available on the genetic diversity of Echinococcus species among livestock in Sudan. In the present study, fifty (n = 50) hydatid cysts were collected from cattle carcasses (one cyst sample per animal) at Al-kadarou slaughterhouse, Khartoum North, Sudan. DNA was extracted from protoscolices and the germinal layer of each cyst and subsequently amplified by PCR targeting the mitochondrial NADH dehydrogenase subunit 1 (NADH-1) gene. The amplified PCR products were purified and subjected to direct sequencing for subsequent construction of phylogenetic tree and net work analysis. Results: The phylogenetic tree revealed the presence of Echinococcus canadenesis genotype 6 (G6) in 44 cysts (88.0%), Echinococcus ortleppi genotype 5 (G5) in 4 cysts (8.0%) and Echinococcus granulosus sensu stricto (s.s) genotype 1 (G1) in 2 cysts (4.0%). The phylogenetic network analysis revealed genetic variation among the different haplotypes/genotypes. This report has provided, for the first time, an insight of the role of cattle in the transmission of the zoonotic G1 echinococosis. Conclusions: The results of the study illustrate that Sudanese breeds of cattle may play an important role in the transmission dynamics and the epidemiology of cystic echinococcosis in Sudan. This study reports the first molecular identification of E. granulosus s.s. in cattle in Central Sudan.背景：细粒棘球绦虫复合群（Echinococcus granulosus sensu lato, s.l.）是囊型包虫病（cystic echinococcosis, CE）的病原体，囊型包虫病是一种世界性人兽共患寄生虫病，可感染人类及包括牛在内的多种哺乳动物。目前关于苏丹家畜中棘球绦虫的遗传多样性的相关研究信息较为匮乏。本研究于苏丹北喀土穆阿尔卡达鲁屠宰场，从牛胴体中采集了50份棘球蚴囊样本（每头动物仅采集1份囊样本）。从每个囊的原头蚴和生发层中提取DNA，随后以线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基1（mitochondrial NADH dehydrogenase subunit 1, NADH-1）基因为靶标进行聚合酶链式反应（PCR）扩增。将扩增得到的PCR产物纯化后进行直接测序，用于后续构建系统发育树与系统发育网络分析。 结果：系统发育树分析显示，44份囊样本（占比88.0%）为加拿大棘球绦虫（Echinococcus canadensis）基因型6（G6），4份（占比8.0%）为奥特普棘球绦虫（Echinococcus ortleppi）基因型5（G5），2份（占比4.0%）为细粒棘球绦虫狭义群（Echinococcus granulosus sensu stricto, s.s.）基因型1（G1）。系统发育网络分析揭示了不同单倍型/基因型间存在遗传变异。本报告首次阐明了牛在人兽共患G1型包虫病传播中的作用。 结论：本研究结果表明，苏丹本地牛种群可能在苏丹囊型包虫病的传播动力学及流行病学过程中发挥重要作用。本研究首次在苏丹中部地区的牛体内完成了细粒棘球绦虫狭义群（E. granulosus s.s.）的分子鉴定。