exosomal ELNAT1 promotes lymphatic metastasis of bladder cancer

The crosstalk between tumor cells and LECs triggers the LN metastasis in bladder cancer (BCa), but the underlying mechanisms are not completely understood. Previously, we identified that BCa-secreted extracellular vesicles (EVs)-packaged lncRNAs mediated the communication with LECs and promoted LN metastasis. To evaluate whether EV-packaged lncRNAs triggered the LN metastasis of BCa, next-generation sequencing (NGS) to the global expression profiles of lncRNAs was utilized in the urinary EVs (urinary-EV) of five MIBC patients and five healthy volunteers. To identify EV-packaged lncRNAs involved in the progression of bladder cancer, NGS was performed in the urinary EVs of five MIBC patients and five healthy volunteers. The RNA from urinary EVs was extracted and enriched with PCR to create the final cDNA library, which was sequenced pair end on the BGISEQ-500/ MGISEQ-2000 System.