Using Illumina MiSeq platform to sequence the partial 16S rRNA library of the hot spring sample

A comprehensive investigation of the microbial community composition and taxonomy of Sungai Klah, which is located in Perak, Malaysia. It is the second hottest geothermal springs in Malaysia. The hot spring is a streamer-like and several spring heads with boiling temperature (100-110 °C) are presence. Other parts of the streaming water exhibit the temperature ranging from 50-100 °C. Little knowledge on the types of hyper- and thermophilic within this hot spring is known. Furthermore, this high temperature environment condition serves as a source for understanding the genetic diversity, population structure, and ecological roles of these boiling point-tolerant microorganisms.