Dataset of cell viability and secreted analytes tumour cell lines treated with low pH and conditioned medium

Extracellular acidity has a profound effect on tumour cell survival and growth, tumour development, and disease progression. Breast and oesophageal squamous cell carcinoma cell lines (MCF-7 and WHCO6, respectively) were treated with medium of decreasing pH, and culture supernatant was removed after 24 hours. Cytokines, chemokines, and growth factors were detected by Luminex. Various levels of apoptosis was induced in acid-exposed cells. This conditioned supernatant was applied to fresh cell cultures for 24 hours and the same proteins were again measured by Luminex. Exposure to conditioned medium had paradoxical effects on both cell lines, with proliferation and survival increasing WHCO6 cells, whereas MCF-7 cells underwent apoptosis. The proteins secreted by these cell lines highlight the differences in survival strategies such as avoiding immune destruction and promoting tumour cell growth. Protein concentrations were determined (pg/ml) in duplicate. One- and two-way ANOVAs followed by post-hoc Dunnett's tests were used for multiple comparisons. Statistical analysis was performed using GraphPad Prism for Windows version 9.4 or 9.5 (California, USA).

细胞外酸性对肿瘤细胞的存活与生长、肿瘤发育以及疾病进展具有深远的影响。本研究中，采用pH值递减的培养基处理乳腺癌和食管鳞状细胞癌细胞系（分别为MCF-7和WHCO6），并在24小时后去除培养上清液。通过Luminex技术检测细胞因子、趋化因子和生长因子。在酸性暴露的细胞中，诱导了不同程度的细胞凋亡。将此条件上清液应用于新的细胞培养中，并在24小时后再次使用Luminex技术检测相同的蛋白质。暴露于条件培养基对两种细胞系产生了矛盾的影响，WHCO6细胞的增殖和存活率增加，而MCF-7细胞则发生了细胞凋亡。这些细胞系分泌的蛋白质突显了在生存策略上的差异，例如避免免疫破坏和促进肿瘤细胞生长。蛋白质浓度以重复测定的形式确定（pg/ml）。通过双因素方差分析（One- and two-way ANOVA）及后续Dunnett事后检验进行多重比较。统计分析采用GraphPad Prism for Windows版本9.4或9.5（美国加利福尼亚州）进行。