Nyvltova et al. MS Data
收藏doi.org2025-01-15 收录
下载链接：
http://doi.org/10.17632/7jsmsm3xrn.1
下载链接
链接失效反馈官方服务：
资源简介：
Nyvltova-A
MS analysis of proteins immunoprecipitated from wild-type HEK293T mitochondrial extracts with antibodies against COX11, PET191, and COX19. Rabbit IgG was used as the negative control. A1-Analysis (original raw data in Scaffold). A2- Samples Report.
Nyvltova-B
MS analysis of proteins immunoprecipitated with beads conjugated with a FLAG antibody (anti-DYDDDDDK beads, Sigma), from mitochondrial extracts of COX11-KO + COX11-FLAG, PET191-KO + PET191-FLAG, and COX19-KO + COX19-FLAG. WT HEK293T was used as the negative control. The mitochondrial samples were treated with crosslinker DSP prior to extraction and immunoprecipitation. Two independent repetitions were performed.
Nyvltova-C
MS analysis of proteins immunoprecipitated with beads conjugated with a FLAG antibody (anti-DYDDDDDK beads, Sigma), from mitochondrial extracts of COX11-KO + COX11-FLAG, PET191-KO + PET191-FLAG, and COX19-KO + COX19-FLAG. WT HEK293T was used as the negative control. Two independent repetitions were performed.
Nyvltova-D
MS analysis of proteins immunoprecipitated from wild-type HEK293T mitochondrial extracts with antibodies against COX11, PET191, and COX19. Rabbit IgG was used as the negative control.
{'Nyvltova-A': '对野生型HEK293T线粒体提取物中经COX11、PET191和COX19抗体免疫沉淀的蛋白质进行MS分析。以兔IgG作为阴性对照。A1-分析（原始原始数据存放在Scaffold中）。A2-样品报告。', 'Nyvltova-B': '对COX11-KO + COX11-FLAG、PET191-KO + PET191-FLAG和COX19-KO + COX19-FLAG的线粒体提取物中，用偶联有FLAG抗体（anti-DYDDDDDK beads，Sigma）的磁珠进行的蛋白质免疫沉淀的MS分析。以野生型HEK293T作为阴性对照。在提取和免疫沉淀之前，线粒体样品经交联剂DSP处理。进行了两次独立的重复实验。', 'Nyvltova-C': '对COX11-KO + COX11-FLAG、PET191-KO + PET191-FLAG和COX19-KO + COX19-FLAG的线粒体提取物中，用偶联有FLAG抗体（anti-DYDDDDDK beads，Sigma）的磁珠进行的蛋白质免疫沉淀的MS分析。以野生型HEK293T作为阴性对照。进行了两次独立的重复实验。', 'Nyvltova-D': '对野生型HEK293T线粒体提取物中经COX11、PET191和COX19抗体免疫沉淀的蛋白质进行MS分析。以兔IgG作为阴性对照。'}
提供机构：
Mendeley Data
