Paul S. Maddox, Francie Hyndman, Joost Monen, Karen Oegema, Arshad Desai (2011) CIL:28776, Caenorhabditis elegans, embryo. CIL. Dataset

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of CeCENP-A by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migration is normal, but chromosome condensation is disrupted. Chromosomes fail to form a proper metaphase plate, and the spindle poles move away from the chromatin masses prematurely. Anaphase segregation fails, and chromatin masses are left in the middle of the cell. The video is ∼19 min long, and the frame is ∼33 µm top to bottom. Images were acquired at 10-s intervals, and corresponding data are shown in Fig. 1A in J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.

对经过RNA干扰去除CeCENP-A的秀丽隐杆线虫胚胎第一次有丝分裂进行旋转盘共聚焦时间间隔成像。GFP-组蛋白和GFP-γ-微管蛋白被成像（品系TH32）。核仁迁移正常，但染色体凝聚受到破坏。染色体未能形成适当的中期板，纺锤体极提前从染色质团块中移开。后期分离失败，染色质团块留在了细胞中央。视频时长约为19分钟，帧高从上至下约为33微米。图像以每10秒间隔采集，相关数据如图1A所示于《细胞生物学杂志》（J Cell Biol.）2007年3月12日第176卷第6期，页码757-763。电子版发表于2007年3月5日。