DualMesoSPIM whole mouse-heart cytoarchitecture dataset: fused autofluorescence tomograms, fibre-orientation meshes, and sympathetic innervation tomogram

This dataset accompanies the publication: Giardini F., Palandri C., et al. "High-throughput, high-fidelity reconstruction of whole mouse-heart cytoarchitecture at cellular scale." Journal of Physiology (submitted). The dataset contains three components: 1. Whole_hearts_tomograms/ (11 files, ~46 GiB)Fused autofluorescence tomograms of 11 adult wild-type mouse hearts (CTRL), acquired using a custom dual-camera selective plane-illumination microscope (dual-mesoSPIM) after CUBIC tissue clearing. Each tomogram was generated by automated dual-view fusion combining the highest-contrast information from two opposing detection arms. Files are 16-bit TIFF stacks. Voxel size: 6.0 × 6.0 × 6.0 µm³ (isotropic, resampled from raw acquisition voxel size of 3.25 × 3.25 × 3.0 µm³). These tomograms were used for Structure Tensor Analysis (STA) of cardiomyocyte fibre orientations and laminar sheetlet organisation. Naming convention: D1.tif – D11.tif. 2. Anatomical_meshes/ (5 files, ~2 GiB)Volumetric tetrahedral meshes of the ventricular myocardium for 5 hearts (subset of the above, selected on the basis of >95% analysable fibre orientation coverage). Each mesh integrates locally prevailing cardiomyocyte fibre orientation vectors (helix angle α, sheet angle β, intrusion angle γ) mapped onto mesh elements, derived from STA applied within non-overlapping macrovoxels of 96 × 96 × 96 µm³ (FA threshold ≥ 0.25). Files are in VTK format. Naming convention: N1.vtu – N5.vtu. 3. AntiTH_tomogram/ (1 file, ~24 GiB)Fused whole-heart tomogram from anti-tyrosine hydroxylase (TH) immunofluorescence imaging of the cardiac sympathetic nervous system, acquired at full acquisition resolution. Voxel size: 3.25 × 3.25 × 3.0 µm³. The tomogram was used for machine-learning-based segmentation of sympathetic axons (Trainable Weka 3D, Fiji) to reconstruct innervation density throughout the intact ventricular myocardium. File: AntiTH_fused_ps_zyx_3.0_3.25_3.25_um.tif. Microscope control and fusion software: https://github.com/giardini-iekm/dual-mesospim.git