Gene expression analysis of HepG2 cells overexpressing TAF2

Taf2 is a transcription co-factor and a member of TFIID complex. To better understand gene regulation of Taf2 we generated stable clones of HepG2 cells overexpressing either eGFP or TAF2-V5. The analysis shows that overexpression of TAF2 activates several pro-tumorigenic pathways and inhibits tumor suppressor pathways. Overall design: RNA was isolated from exponentially growing cells and subjected to RNA-seq analysis.