Mapping of genome-wide DSBs in HEK293T cells using the RAFT procedure.. Homo sapiens
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA233403
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资源简介：
Large DNA fragments (mainly of 50-250 kb, denoted as forum domains) were isolated inside agarose plugs as described below in the extract protocol section. After ligation of 5'-bio-oligos to the blunt DNA termini the digestion with Sau3A enzyme was performed followed by selection of genome-wide preparation of forum domains termini on streptavidin-paramegnetic particles. After ligation of Sau3A adapter the termini were PCR amplified and used for deep sequencing. Overall design: Mapping of Double-Strand DNA Breaks/Forum Termini
创建时间：
2014-01-03
