Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Embryonic Fibroblasts (MEFs) with LRRK2 inhibition.

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Embryonic Fibroblasts (MEFs) with LRRK2 inhibition.". Description: Quantitative DIA-based proteomic analysis of isolated lysosomes (Lyso-IP) and whole-cell extracts (WCL) from VPS35[D620N] knock-in MEFs. The dataset includes six biological replicates comparing cells treated with the LRRK2 inhibitor MLi-2 (100 nM, 2 h) against DMSO vehicle controls. Expression of the 3XHA-TMEM192 lysosomal tag was determined by genotyping PCR and verified via anti-HA immunoblotting. Data acquired on an Orbitrap Exploris 480 and processed using DIA-NN 1.8.1. This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset