Primer Sequences.

(A) RT-PCR primers for verifying AKAP13 gene-trap splicing events are listed. The primer locations and mutant line verified are indicated. The size of the RT-PCR product is given in base pairs (bp). (B) The common long-range PCR primers within the gene-trap construct are listed. These primers were used with AKAP13 specific genomic DNA primers to identify the gene-trap insertion. (C) The genotyping primers used to identify the wild-type and mutant allele for the three mutant mouse lines are listed. The primer direction is also given: forward (For) and reverse (Rev). The size of the PCR product is given in base pairs (bp).