Gene Level Expression Profiling of Cardiac Progenitor Cells and Cardiomyocytes

Transcriptome analysis of cardiac progenitor cells and cardiomyocytes The identification of cell surface proteins on stem cells or stem cell derivatives is a key strategy for the functional characterization, isolation, and understanding of stem cell population dynamics. Here, using an integrated mass spectrometry and microarray based approach, we analyzed the surface proteome and transcriptome of cardiac progenitor cells (CPCs) generated from the stage-specific differentiation of mouse and human pluripotent stem cells. We analyzed 9 samples from differentiated mouse embryonic stem cells using the Affymetrix Mouse Exon 1.0 ST platform. Array data was processed by the Transcriptome Analysis Console (TAC) Software. No technical replicates were performed.