Dataset of microscope images of prefrontal cortex from wistar rat tissue after an induced stroke

Microscopic images were captured with the Leica ICC50HD camera mounted on the Leica DM500 microscope, performed with fixed parameters in magnification 20x, and 56% horizontal and 40% vertical overlap. The rows and high-resolution images was generated using the captured images and an stitched algorithm using the homography matrices. The homography matrices were obtained from a small number of correspondences manually selected between each pair of images horizontal displacements and between pair of rows in vertical displacement. Raw microscope images PNG format format. High-resolution image PNG format , pixel size 0.3 µm, area 92.48 mm2. Homography matrices HDF5 files Dataset organization Folder -> Filename -> Description \rawImages\ -> image_Row_RR_XX.png -> Images of tissue numbered as XX (varies each row: 01 up to 45) of images rows numbered as RR (01-36) \stitched\tissue\ -> brainTissueImage.png -> High-resolution obtained. \stitched\rows -> image_Row_XX.png -> Images for each row numbered as RR (01-36). \homographies\ -> homography_images_Row_RR.h5 \homographies\ -> homography_Rows.h5 -> Homography matrices between each pair of images per row numbered as RR (01-36).本数据集的显微图像通过搭载于徕卡DM500显微镜的徕卡ICC50HD相机拍摄，拍摄参数固定为20倍放大倍率，水平重叠率56%、垂直重叠率40%。利用拍摄得到的单幅图像与基于单应矩阵（homography）的拼接算法，生成了单排拼接图像与高分辨率整体图像。 上述单应矩阵通过手动选取每幅图像间水平位移对应点、以及每排图像间垂直位移对应点的少量匹配点对得到。 原始显微图像采用PNG格式存储；高分辨率图像同样为PNG格式，像素尺寸0.3 µm，成像面积92.48 mm²；单应矩阵以HDF5文件格式存储。 数据集组织规范如下： - awImages 目录下的 image_Row_RR_XX.png：对应编号为RR（01至36）的图像排中，编号为XX（01至45，每排编号范围各有不同）的组织显微图像。 - stitched issue 目录下的 brainTissueImage.png：最终生成的高分辨率脑组织拼接图像。 - stitched ows 目录下的 image_Row_RR.png：对应编号为RR（01至36）的单排拼接图像。 - homographies 目录下的 homography_images_Row_RR.h5：对应编号为RR（01至36）的单排内各图像间的单应矩阵文件。 - homographies 目录下的 homography_Rows.h5：各排图像之间的单应矩阵文件。