Understanding the multicellular dynamics of clear cell renal cell carcinoma DNA sequencing

This study will reconstruct the single cellular phylogeny of tumour and immune cells in relation to both genomic, functional, and spatial location. 20 tumours in total will be studied, including non-progressing small renal masses, progressing small renal masses, relatively indolent larger tumours, and high risk tumour with metastatic sampling. We aim to:

Generate a focused, comprehensive phylogenetic characterisation of tumour cells
Understand the dependencies of the cancer genome, transcriptome, tissue architecture and the single cellular micro-environment
Quantify the single cellular composition and functional intra- and inter- tumoural heterogeneity
Determine the phylogeny of tumour associated lymphocytes and their relationship to neo-epitopes and the immune microenvironment
Investigate the mechanisms by which the tumour-normal interface constrains tumoural growthEGA study EGAS00001003517