Integration of transcriptome and proteome analyses reveiled molecular mechanisms for formation of replant disease in Nelumbo nucifera

The normal growth of Nelumbo nucifera, a widely planted aquatic crop in Asia, was severely ruined by replant disease. To investigate changes of transcripts in response to replant disease, transcriptomic analyses were performed by comparing seedings of first-year planting (FP) and consecutive planting (CP). 9810 differentially expressed genes (DEGs) were identified between FP and CP. Based on the biological function of these target genes, the signal pathway of consecutive monoculture was illustrated in N.nucifera. The experiments aimed to provide data for unveiling the molecular characteristics of replant disease, and explore our understanding of replant disease in aquatic botony. mRNA profiles of first-year planting group (FP) and consecutively planting group (CP) in rhizomes of N.nucifera were generated by deep sequencing, in triplicate.