Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-4)

Annotation description: Dense segmentations of nuclei in jrc_mus-nacc-4 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.
Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)
Software: Amira 3D 2021.1, Classic Segmentation Workroom
Classes annotated: Nuclei
Source (EM) dataset ID: jrc_mus-nacc-4
Source (EM) voxel size (nm): 4 x 4 x 4 (X, Y, Z)
Source (EM) data dimensions (µm): 20.3 x 20.5 x 3.0 (X, Y, Z)
Source (EM) DOI: https://doi.org/10.25378/janelia.24222988
Dataset URL: s3://janelia-cosem-datasets/jrc_mus-nacc-4/jrc_mus-nacc-4.zarr/recon-2/labels/inference/segmentations/nuc/
Visualization website: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-4