The initial age-associated decline in early T-cell progenitors reflects fewer pre-thymic progenitors and altered signals in the bone marrow and thymus microenvironments

To identify candidatemechanisms underlying the early decline in CLPs and MPPs by 3MO. Overall design: We carried out bulk RNA-seq on 1MO v 3MO CLPs and MPPs (N=3 independent experimental repeats) using 150bp paired-end sequencing with a target of 20 million reads per sample.