Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12173, Gallus gallus gallus, memory B cell. CIL. Dataset
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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP-OFF cells expressing mutant TrAP-INCENPF802A,. See Fig 6C panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞核横断面。采用Deltavision系统,通过100x/1.4NA计划阿波卡镜头捕获的宽场免疫荧光图像序列,并利用约束迭代算法(Applied Precision)进行反卷积处理。展示的是中央的Z切片。在表达突变型TrAP-INCENPF802A的INCENP-OFF细胞中,观察到外源性的TrAP-INCENP(红色)定位于中期,内源性的CENP-A(绿色)和DNA(蓝色)。详情请参见Xu等人在2009年《细胞生物学杂志》(J Cell Biol)第187卷的第637至653页的第6C部分第13面板。
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CIL



