Changes in the mRNA levels of Hif1a, Hif2a and several HIF-target genes in islets isolated from diabetic mice.

Gene/Cyclophilin mRNA ratio were measured by real-time PCR using islet cDNA from 10-week-old diabetic Leprdb/db mice and their non-diabetic Leprdb/+ littermates (fed whole blood glucose levels ∼20 mmol/l and ∼6 mmol/l respectively)(primer sequences are shown in Table S3). The metabolic characteristics of the mice and the mRNA levels of other β-cell genes (including Hk1 and Ldha) in these series of experiments have been reported earlier [26]. Data are means ± SE for 5–9 mice and are expressed relative to the mRNA ratio in control Leprdb/+ mice.apvs. control by Student t-test.