Effects of REDD1 KO in transcriptomic response to wounding and glucocorticoid treatment in HaCaT human keratinocytes

REDD1 KO affects basal global gene expression profile and transcriptomic response to wounding and glucocorticoid treatment in HaCaT human keratinocytes Overall design: REDD1 KO HaCaT human keratinocytes were obtained with Crispr/Cas9 technology. The KO and WT (Cas9 only) HaCaT human cells were grown to 90% confluency and treated with either vehicle (WT or KO), 1uM glucocorticoid floocinolone acetonide (FA) (WT_FA or KO_FA), or wounded by scratching (WT_Wound or KO_Wound), or wounded and treated with FA (WT_Wound_FA or KO_Wound_FA), with all conditions incubated for 24 h before harvesting.