Frequencies of outcomes after an I-<i>Sce</i>I-induced DSB with different <i>S. cerevisiae</i> chromosome III configurations.

#Data from Table 1, repeated here for convenient reference. a–e, j–l,nSee Table 1 for details. fS. cerevisiae chromosome III with wild-type Ty configuration is designated as “None”. gTy gene conversion (GC) events have a Leu+HygSUra+ phenotype with a wild-type S. cerevisiae chromosome III size. However, a small fraction of Ty GC events in purebreds have a Leu+HygSUra− phenotype (see s), most likely the result of gene conversion with crossover. In hybrids, Ty GC events are NAHR events since repair must use a non-allelic Ty donor from S. cerevisiae genome. In purebreds, we cannot distinguish if Ty GC events are through NAHR since an allelic or non-allelic Ty donor may be used. However, it is likely that the majority of Ty GC repairs using an intrachromosomal Ty donor (Roeder et al., 1984), making these NAHR events. hDeletion (Del.) contained within RAHS locus (“intra”); DSB is flanked by 1,571 bp of perfect identity in RAHS cluster. iDeletion between two different Ty loci (“inter”), RAHS and FRAHS; labeled “inter-Ty” here to distinguish between intra-Ty deletions; inter-Ty is the same as internal deletions in Table 1. mI-SceI cut site/HYG construct inserted at SGD coo. 163491; DSB is 11,657 bp from RAHS, 28,874 from YCRCdelta7, and 47,488 bp from YCRCdelta6. oNo Loss SEM, genetic analysis from one experiment. pI-SceI cut site/HYG construct inserted at SGD coo. 151931; DSB is 97 bp from RAHS, 17,314 bp from YCRCdelta7, and 35,929 bp from YCRCdelta6. qI-SceI cut site/HYG construct inserted in position 5310 in RAHS Genbank accession GU220389 (TyB-POL of YCRWTy1-2). rI-SceI cut site/HYG construct inserted at SGD coo. 147932; DSB is 4,869 bp from YCRCdelta7 and 23,483 bp from YCRCdelta6 s30.6±8.8 are Leu+HygSUra+ and 2.6±0.6 are Leu+HygSUra−. *Compiled from strain MH3524, MH3572, MH3573; same strain transformed with different I-SceI plasmids (see Table 1). n.a., not applicable. Ty locus deletion(s) result in the absence of the corresponding rearrangement size class by PFGE/Southern analysis. Ty GC and Intra-Ty events occur with DSB in repetitive sequences at RAHScs, but not with DSB in unique sequences (147cs, 151cs, 163cs). n.d., not determined in purebreds since cannot distinguish between allelic or non-allelic Ty donor for Ty GC category. DSB, double-strand break; SEM, standard error of the mean; NAHR, non-allelic homologous recombination; Isochr., isochromosome; Trans., translocation; Chr. loss, chromosome loss; cs, cut site; LAHS, left arm transposition hotspot; RAHS, right arm transposition hotspot; FRAHS, far right arm transposition hotspot.