Real-time quantitative PCR analysis of inflammation-related genes of organs and tissues from mice in response to Coxsackievirus A2 (CVA2) infection

For in vivo experiments, mice were inoculated a lethal dose of CVA2 strain (104 TCID50/mouse) or equal volume of culture supernatant of RD cells that were uesd to cultivate virus. Total RNA was extracted from brains, lungs, hearts and skeletal muscle of mice at 3 days post infection (dpi), 5 dpi and corresponding controls using TRIzol reagent to quantitate inflammation-related genes from the CVA2 infected mice and control. qPCR gene expression profiling. Brains, lungs, hearts and skeletal muscles from four groups were treated separately. Equal amount total RNA from each organ or tissue of mice was harvested to analyze inflammation-related genes. There were 4 duplicate samples per group and 4 different organs or tissues per mouse. Equal amount total RNA was extracted from 64 samples.