Gene expression changes associated with KIAA1199 expression in SW480 cells. Homo sapiens
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA171151
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资源简介：
The aim was to identify gene expression changes upon expression of KIAA1199 in a colon cancer cell line. Overall design: Inducible expression of KIAA1199A was achieved with a two-step procedure. First, SW480 cells were transfected with the pcDNA6TR vector (Invitrogen) and the tetracycline repressor-expressing cells selected with 7 μg/ml blasticidin S (InvivoGen). The blasticidin-resistant cells were then transfected with the pT-Rex-DEST30-KIAA1199 construct (see below) and selected with 0.4 mg/ml G418. The growth medium used for these cells was supplemented with 10% Tet-system-approved fetal calf serum (Biochrom).
创建时间：
2012-07-23
