Spatial transcriptomic analysis of human dorsoal root ganglia neurons

Xenium platform was used for the spatial transcriptomic analysis of human
DRG neurons, 100 marker genes were selected as the customized probe panel
and hybridized to fresh frozen hDRG sections. Manual segmentation of each
neuron soma was performed, based on expressions of pan-neuronal marker
gene PGP9.5, satellite glia cell marker FAB7B, and the corresponding H.E.
staining. In total, 1340 neurons were identified (excluding 75
region-of-interest with poor or unclear neuronal soma morphology in H
& E staining) and clustered into 16 groups. The 16 clusters were
assigned as different cell types based on marker genes expression.