Split-GFP data for experimentally addressed Chroma proteins

Raw Nanopore sequencing read counts from FACS-sorted cell populations and enrichment score calculations. Bin scores were calculated for each protein as detailed in the Supplemental Methods section. [L1, L2, L3] refer to experimental replicate IDs, and [G0, G1, G2, G3] refer to sorting bin IDs. PRE refers to pre-sort populations. N is normalized read counts within each bin, an ENR is read enrichment for each bin compared to PRE. Bin scores were averaged for duplicate or triplicate biological replicate experiments, as indicated. Secondary structure information for each protein also included: G = 3-turn helix (310 helix). Min length 3 residues; H = 4-turn helix (α helix). Minimum length 4 residues; I = 5-turn helix (π helix). Minimum length 5 residues; T = hydrogen bonded turn (3, 4 or 5 turn); E = extended strand in parallel and/or anti-parallel β-sheet conformation. Min length 2 residues; B = residue in isolated β-bridge (single pair β-sheet hydrogen bond formation); b = also isolated bridge bend (the only non-hydrogen-bond based assignment); C = coil (residues which are not in any of the above conformations).