Transcriptome analysis of C1q-administrated murine lungs

Pulmonary fibrosis (PF) is a progressive fibrotic disease with a poor prognosis and suboptimal therapeutic options. The complement molecule C1q, which plays an important role in the phagocytic capacity of macrophages, has recently been reported to exacerbate several fibrosis-related diseases. On the other hand, there are still no reports in PF. Here, we analyzed gene expression changes of C1q-administrated murine lungs. To clarify transcriptomic landscape of C1q-administrated murine lungs, we intratracheally administered with C1q (20 µg/mouse) to WT mice and lungs were harvested. Lung cells were performed transcriptome analysis by 5′sequence using Illumina Novaseq 6000 sequencer.