Positive-going hybrid indicators for voltage imaging in excitable cells and tissues

Membrane potential dynamics are crucial to the physiology of electrically excitable cells, such as neurons, cardiomyocytes, and pancreatic islet cells. Fluorescent voltage indicators have enabled the visualization of electrical activities at the single-cell level across large cell populations. However, most existing indicators have a negative fluorescence-voltage response, resulting in a high background signal when cells are at their resting state. To reduce background interference during voltage imaging, we developed a positive-going hybrid voltage indicator (HVI+), which has a lower intensity at the resting state and becomes brighter upon membrane depolarization. HVI+-Cy3b exhibits a remarkable voltage sensitivity of 55% ΔF/F0 per 100 mV, enabling accurate reporting of action potential waveforms in beating cardiomyocytes and neurons. Additionally, by combining HVI+-Cy3b with HVI-Cy3b, we demonstrate the capability to simultaneously assess the effects of glucose on the spike activities..., , , # Positive-going hybrid indicators for voltage imaging in excitable cells and tissues

Dataset DOI: [10.5061/dryad.m63xsj4dd](10.5061/dryad.m63xsj4dd)

## Description of the data and file structure

We have submitted the codes for voltage imaging (LabVIEW code folder) and data analysis (MATLAB code folder) used in the paper \"Positive-going hybrid indicators for voltage imaging in excitable cells and tissues\".

### Files and variables

#### File: Code_for_voltage_imaging_with_HVI_and_HVIplus.zip

**Description:** **This file contains LabVIEW code for voltage imaging and MATLAB code for data analysis.**

**The analysis codes include two parts: analysis in HEK293T and analysis in excitable cells or tissues.**

**Analysis in HEK293T cells:**

1\) Kinetics and sensitivity analysis for negative-going indicator: for HVI and other negative-going voltage indicators with an overshoot depolarizing (Table S2).

2\) Kinetics and sensitivity analysis for positive-going indicator: for HVIplus and othe...,